Welcome to the Gilkes Lab

Breast & Gynecologic Malignancies Group   Johns Hopkins School of Medicine

Gilkes Lab Christmas Lunch in 2023


Gilkes Lab

Our lab is part of the Breast & Gynecologic Malignancies Group at the Johns Hopkins School of Medicine. Our work is focused on the role of hypoxia in breast cancer metastasis. One of our main interests are the gene expression changes that happen under hypoxia and how they can both contribute to tumor progression. We are a dynamic and creative lab that always likes a good challenge. We address our questions through 2D and 3D in vitro, and in vivo systems.

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Learn more about hypoxia and metastasis here

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Learn more about our involvement with the The Johns Hopkins Breast Cancer Research Advocacy Program here

Click here to see the most recent updates, news, and events from our lab!



Daniele Gilkes

Daniele Gilkes, PhD

Principal Investigator

Daniele M. Gilkes is an Assistant Professor in the Oncology Department, under the Breast and Ovarian Cancer Program, at Johns Hopkins School of Medicine. She earned B.S.ChE and M.S. degrees in the University of Florida. After completing her PhD in Cancer Biology at the University of South Florida, Daniele became a Susan G. Komen Postdoctoral Fellow in the lab of Gregg Semenza, MD, PhD at Johns Hopkins School of Medicine. Later, she became a Research Assistant Professor in the lab of Denis Wirtz, PhD in the Chemical & Biomolecular Engineering Department at Johns Hopkins University. Daniele opened her lab in 2015, and now focuses on studying the role of the tumor microenvironment, primarily hypoxia and the extracellular matrix, in breast cancer.

Harsh with Daniele Gilkes
Harsh Oza

Research Technologist

Harsh Oza is currently working on expanding his Master's thesis project in the lab as a Research Technologist. He earned his Master’s degree from Johns Hopkins University, Department of Biotechnology in May 2021. He received a Bachelor's in Biotechnology from D. Y. Patil University, Mumbai, India in the year 2018. He previously worked on cancer research and the study of hypoxia at Advanced Centre for Treatmemt, Research and Education in Cancer [ACTREC]: Tata Memorial Centre [TMC] in India.

Yi Shi with Daniele Gilkes
Yi Shi

Post-doctoral Fellow

Yi Shi is postdoctoral fellow in the lab. She is working on identifying how Intratumoral hypoxia promotes metastatic liver and brain metastatic-organotropism in breast cancer.

Esther with Daniele Gilkes
Esther Ng

Undergraduate Student

Esther Ng is an undergraduate at Johns Hopkins University majoring in Molecular & Cellular Biology and Public Health. She plans to attend medical school in the future.

Daniele Gilkes

Research Technologist

Swathi Talluri her Masters in Molecular & Cellular Biology and is currently a Research Technologist in the lab.

Daniele Gilkes

Master's Degree student

Jingyuan is a Master’s Student in the Department of Biochemistry and Molecular Biology Department in the Bloomberg School of Public Health.

Daniele Gilkes

Undergraduate Research Assistant

Undergraduate student majoring in Chemical & Biomolecular Engineering at Johns Hopkins University

Daniele Gilkes

Undergraduate Research Assistant

Undergraduate student majoring in Chemical & Biomolecular Engineering at Johns Hopkins University

Reid with Daniele Gilkes

Undergraduate Research Assistant

Undergraduate student majoring in Chemical & Biomolecular Engineering at Johns Hopkins University

Click here to see a list of our former members and what they're up to now!


Uncovering the spatial landscape of molecular interactions within the tumor microenvironment through latent spaces

Mebendazole Treatment Disrupts the Transcriptional Activity of Hypoxia-Inducible Factors 1 and 2 in Breast Cancer Cells

Mebendazole prevents distant organ metastases in part by decreasing ITGβ4 expression and cancer stemness

Extracellular fluid viscosity enhances cell migration and cancer dissemination

The Rate of Cisplatin Dosing Affects the Resistance and Metastatic Potential of Triple Negative Breast Cancer Cells, Independent of Hypoxia

Multi-compartment tumor organoids

Valsartan and sacubitril combination treatment enhances collagen production in older adult human skin cells

Detection of Hypoxia in Cancer Models: Significance, Challenges, and Advances

A persistent invasive phenotype in post-hypoxic tumor cells is revealed by fate mapping and computational modeling

A common goal to CARE: Cancer Advocates, Researchers, and Clinicians Explore current treatments and clinical trials for breast cancer brain metastases

Post-Hypoxic Cells Promote Metastatic Recurrence after Chemotherapy Treatment in TNBC

Hypoxia-inducible factor-dependent ADAM12 expression mediates breast cancer invasion and metastasis

Extracellular matrix-bound FGF2 mediates estrogen receptor signaling and therapeutic response in breast cancer

Hypoxia alters the response to anti-EGFR therapy by regulating EGFR expression and downstream signaling in a DNA methylation-specific and HIF-dependent manner

Single-cell morphology encodes metastatic potential

Fate-mapping post-hypoxic tumor cells reveals a ROS-resistant phenotype that promotes metastasis

Hypoxia and Cancer Metastasis (Textbook)

Solid Stress in Brain Tumors

The Contribution of the Immune System in Bone Metastasis Pathogenesis

RhoB is regulated by hypoxia and modulates metastasis in breast cancer

A software tool for the quantification of metastatic colony growth dynamics and size distributions in vitro and in vivo

Molecular Portrait of Hypoxia in Breast Cancer: A Prognostic Signature and Novel HIF-Regulated Genes

The Biophysics of 3D Cell Migration

Tumor Hypoxia As an Enhancer of Inflammation-Mediated Metastasis: Emerging Therapeutic Strategies

The quaternary state of polymerized human hemoglobin regulates oxygenation of breast cancer solid tumors: A theoretical and experimental study

RhoB: Team Oncogene or Team Tumor Suppressor?

Hypoxia Selectively Enhances Integrin a5B1 Receptor Expression in Breast Cancer to Promote Metastasis

BRCA1 and BRCA2 mutations and treatment strategies for breast cancer

Hypoxia-Inducible Factors & Cancer

Tumor Mechanopathology: Cutting the Stress Out

Implications of Hypoxia in Breast Cancer Metastasis to Bone

Feel free to contact us

General Inquiry: gilkeslab@gmail.com

Direct Correspondence: dgilkes1@jhu.edu

Twitter: @GilkesLab

Breast and Gynecologic Malignancies Program

1650 Orleans Street

CRBI Room 128

Baltimore MD, 21231


If you are interested in supporting our lab's research, gifts are accepted here.